Hepatokines and Adipokines in Metabolic Syndrome

Introduction

Metabolic syndrome (MetS) is a collection of related health issues that has a close association with T2D and cardiovascular diseases. It has turned into an epidemic disorder and describes many clinical and metabolic risk markers. MetS comprises abdominal adiposity and dyslipidemia characterized by insulin resistance, inflammation, low high density lipoprotein (HDL) cholesterol, high triglycerides, and blood pressure, which also correlate with cardiovascular disease (CVD).^1,2

MetS has been defined in various aspects by the World Health Organization (WHO). Other groups working on epidemiology, endocrinology, basic science, public health have also proposed their definition on MetS.^3,4,5 In the present day definition, MetS has been framed according to NCEP-ATP III and other clinical and biochemical parameters.

Studies have shown a varying prevalence of MetS in people above the age of 20 years, ranging from around 20% to 39.3% in India, the USA, Tehran, Turkey and Saudi Arabia.^6,7 MetS is becoming more common due to an increase in the occurrence of obesity and may overtake smoking as the main cause behind heart disease in future.

The major risk markers of metabolic syndrome are described below and mentioned in ►Fig. 1.

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Fig. 1

Major risk markers of metabolic syndrome.

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• 1. Adiposity

Being overweight and deposition of abdominal fat is the root cause of MetS. Body fat deposits in different regions in females and males following separate patterns. These tendencies of fat deposition are called gynoid and android adiposity because women tend to deposit excess adipose tissue in outlying regions like legs, and pelvic region while men have tendencies to deposit adipose tissue in the central region (like abdomen). However, the position of adipose tissue is very complex because the regions of body fat deposition are common in men and women. Notably, deposition of adipocytes in abdominal region creates more complexities and lead to MetS. It leads to glucose intolerance, where the action of insulin is shunted.⁸

• 2. Insulin resistance

Insulin resistance (IR) is a key factor in MetS, linking the other components stated above. These metabolic abnormalities are the outcomes of the interaction between glucose utilization and storage in skeletal muscles and adipose tissue, culminating in increased insulin demand and hyperglycemia. Healthy women and men have fat content between 18% and 20% and 10 and 15% of body weight, respectively. MetS affects 30 to 40% of people, mainly due to an increase in weight, and intra-abdominal or ectopic fat accumulation. Reducing weight by 5 to 7% by diet and workout can lower MetS, risk of type 2 diabetes, and cardiovascular diseases. Moreover, anti-diabetic drugs that can improve insulin resistance, reduce the level of lipid and control weight gain are helpful for people with type 2 diabetes arising out of MetS. Bariatric surgery is another option for those with body mass index (BMI) greater than or at least 40 kg/m² or 35 to 40 kg/m² with significant co-morbidities.⁹

• 3. Type 2 Diabetes (T2D)

The possibility of T2D increases by five times in MetS patients. MetS and insulin resistance together have a cumulative effect because these patients are more prone to develop T2D. T2D is often linked to chronic inflammation. MetS in females, who have pregnancy-induced diabetes, also significantly escalates the likelihood of developing T2D.¹⁰

• 4. Dyslipidemia

In insulin-sensitive individuals, insulin inhibits adipose tissue lipoprotein lipase. Insulin resistance therefore enhances lipolysis in adipose tissue, resulting in FFA overproduction, increasing its plasma level and also uptake by hepatocytes. FFA increases liver triglyceride and cholesterol ester content. High level of triglycerides in blood TG content induces function of cholesterol ester transfer protein (CETP), that in turn stimulates removal of triglyceride from very low-density lipoprotein (VLDL) and adding to high-density lipoprotein (HDL), hence HDL clearance increases. It also stimulates the conversion of TG into LDL. LDL again in turn undergoes lysis, resulting in the formation of tiny and concentrated cholesterol less LDL particles, which increases the risk of cardiovascular diseases.¹¹

• 5. Hypertension

Hypertension has become a global pandemic, developing countries facing the brunt of the problem, where the number of hypertensives are estimated to increase by 80% by 2025.¹² Approximately 1 billion people are affected globally by hypertension and associated heart and kidney disease. It is the one of the major causes behind global mortality. Hypertension is intricately linked to insulin resistance, high cholesterol, cardiovascular diseases, and MetS. Hyperinsulinemia or the increased insulin levels promote hypertension as a compensation for lesser insulin-mediated glucose uptake by skeletal myocytes. Moreover, resistin, and leptin secretion from adipocyte increases during hypertension and insulin resistance.^13,14

Adipokines in Metabolic Syndrome

Adipokines have a wide variety of role in multiple physiological functions leading to abnormal characteristics of MetS. Anomalous production of adipokines from visceral fat contribute to a proinflammatory condition. An interplay of low adiponectin and high leptin and resistin contribute to dyslipidemia, hyperinsulinemia, and atherosclerosis. To date, only leptin has been developed into a drug.^15,16 Dipeptidyl peptidase-4 (DPP-4) has been recognised as an adipokine and DPP-IV inhibitors (gliptins) are already in use for the treatment of type 2 diabetes.^17,18 Extensive research is needed to identify the basic mechanism of action of adipokines such as apelin, chemerin and resistin. Deficiency in understanding the mechanism of action of these adipokines has retarded the development of adipokine-based therapeutic strategies.¹⁹ Further research is needed to identify the specific adipokines with potential diagnostic and therapeutic properties. The role of different adipokines in MetS are discussed below and shown in ►Fig. 2.

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Fig. 2

Adipokines in metabolic syndrome: role of resistin, IL-6, TNF-α, adiponectin, and leptin in dyslipidemia, insulin resistance, inflammation, type 2 diabetes, and hypertension.

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• 1. Resistin

The most crucial point about resistin is that it counteracts insulin's action. Its expression is obstructed during adiposity as well as in T2D. This inhibition of the expression of resistin is mediated by increased expression of TNF-α. Under normal condition, resistin particularly decreases the repressive action of insulin so that gluconeogenesis can occur in the liver. When resistin does not function, circulatory glucose concentrations decreases and insulin sensitivity improves. The gene expression and secretion of resistin increases during adipogenesis in 3T3-L1, which in turn inhibits 3T3-LI adipogenesis.^20,21 Circulatory resistin remains high in obese and T2D patients.²² However, in certain cases, resistin levels remain quite low in patients having adiposity as well as insulin resistance, which makes the role of resistin controversial. Hyperglycemia is caused by excess secretion of resistin due to increased hepatic glucose production. Resistin also subdues the activation of AMP-activated protein kinase (AMPK) in hepatocytes and muscle cells.^23,24 However, these restrictive actions of resistin upon the activation of AMPK do not occur in primary culture of murine muscle cells, advocating that this process requires release of other factors from other cell types.²⁵ Moreover, the level of resistin in circulation increases with increasing BMI in humans as well as rodents.²⁴ A recent meta-analysis showed circulatory resistin levels to be higher in hypertensive subjects compared to their normotensive counterparts, suggesting a pathogenic role of resistin in hypertension.²⁶ Nevertheless, additional investigation is necessary to identify the detailed function of resistin in MetS.

• 2. Interleukin-6 (IL-6)

Interleukin-6 (IL-6) is considered as a very important adipocytokine that regulates immune responses. It is produced by fibroblasts, monocytes, and adipocytes. Adipocyte-derived IL-6 makes up almost 15 to 35% of total circulating IL-6.²⁷ The receptor of IL-6 functions by regulating other signaling molecules.²⁸ It must be mentioned that plasma concentrations and adipocyte-derived IL-6 levels rise during obesity.²⁸ In high-fat diet (HFD) fed mice, elevated levels of IL-6 in adipocytes induce insulin resistance, inhibiting the function of insulin receptor via proteasomal degradation of insulin receptor substrate (IRS).²⁹ A key component related with IL-6 is C-reactive protein, and their concentrations are positively correlated.³⁰ The presence of IL-6 is found in a wide range in different fat or lipid deposition sites. For example, IL-6 levels in visceral adipocytes are higher than peripheral depositions. Altogether, the relation between IL-6 concentration and T2D is quite complicated. Level of IL-6 in circulation increases in obese and T2D patients with significantly enhanced concentrations in visceral adipocytes.³¹ IL-6 activates AMPK-activated protein kinase in myotubes; however, the detailed signaling pathway involved in IL-6-mediated glucose uptake and other downstream elements in AMPK activation are not known.³² Hence, IL-6, a pro-inflammatory cytokine, can be regarded as an indicator of insulin resistance, adiposity, and T2D.³³

• 3. Tumor Necrosis Factor-alpha (TNF-α)

Just like IL-6, TNF-α is also highly expressed in visceral adipocytes than peripheral adipocytes. It is profusely released by macrophages. TNF-α levels in adipocytes increase with obesity, BMI, insulin resistance, and T2D.³⁴ TNF-α lowers the level of resistin and also impairs its function in 3T3-L1 adipocytes. Blocking of TNF-α affects other adipokines and improves inflammatory markers. It must be mentioned that TNF-α impairs insulin function in liver and adipocytes.³⁵ Incubation with TNF-α affects insulin-mediated glucose uptake in skeletal muscle cells. Moreover, TNF-α receptor-IgG chimeric protein restores phosphorylation and insulin signaling via its receptor and substrate (insulin receptor substrate-1/IRS-1) in adipocytes and skeletal muscle cells. Also, knockout of TNF-α or TNF-α receptor improves glucose homeostasis and increases insulin sensitivity in obese models but not in obese patients with T2D.^36,37 In short, TNF-α levels in adipocytes is positively correlated with fasting plasma glucose levels, insulin, and triglyceride levels in high fat-fed female subjects. It also increases adipocyte lipolysis.³⁸ Hence, TNF-α elevates insulin resistance because it stimulates the production of lipids from adipocytes. Macrophages that are produced from adipose tissue resident monocytes are another significant source of pro-inflammatory cytokines. Also, the ratio of pro-inflammatory and anti-inflammatory cytokines regulate insulin resistance, heart, and kidney diseases.³⁹

• 4. Adiponectin

Adiponectin is a very crucial adipocyte-derived protein because it is a key regulator of obesity and T2D. While it can induce release of proinflammatory cytokines, it also has anti-inflammatory functions and affects nuclear factor (NF-kB). It also enhances the action of insulin in peripheral tissues. Adiponectin decreases glucose concentrations and induces liberation of fat without increasing insulin levels, hence acting as an endogenous insulin sensitizer. Adiponectin is circulation occurs in three forms: trimer, hexamer, and high molecular weight (HMW) 12-18-mer adiponectin.⁴⁰ It enhances energy expenditure in hepatocytes and skeletal muscle cells, while reducing tissue triglyceride content. Adiponectin restores insulin sensitivity through AMPK-induced reduction of gluconeogenesis in the liver and increased glucose uptake by muscles.⁴¹ There are two major receptors for adiponectin-AdipoR1 and AdipoR2, having seven transmembrane domains. AdipoR1 is found in muscles, and AdipoR2 is found in the liver.⁴² Increased plasma level of adiponectin is known to significantly reduce the risk of T2D. High molecular weight (HMW) adiponectin is an important biomarker for MetS, and mainly responsible for increased insulin sensitivity.⁴³ Hypoadiponectinemia is typical in metabolic and cardiovascular disease states such as T2D, non-alcoholic hepatic steatosis, hypertension, and heart disease. Genetic hypoadiponectinemia is mediated by a missense mutation. Decrease in adiponectin levels precede the progression of T2D, making it an important component behind the condition. Adiponectin levels affect the formation of glucose in the liver by decreasing gene expression of enzymes: phosphoenolpyruvate carboxykinase as well as glucose-6-phosphatase. These two enzymes are very important for gluconeogenesis. Adiponectin also aids in the formation of foam cells from macrophages and reduction of TNF-α production from macrophages.⁴⁴ Adiponectin induces weight loss and ameliorates dyslipidemia. Adiponectin expression is reduced in people with metabolically unhealthy adiposity (diabesity).⁴⁵ Increased adipocyte accumulation in the visceral region is linked to several health issues. Interestingly, individuals with higher visceral adipose tissue (VAT) have low level of circulatory adiponectin.⁴⁶ Adiponectin manifests glucose tolerance by insulin-sensitivity, energy utilization, and anti-inflammatory functions. Adiponectin also aids in the development of MetS in women after menopause.⁴⁷ A recent clinical study on post-menopausal women showed that a decrease in body weight significantly increased adiponectin levels and reduced insulin in circulation in fasting condition and also IR.⁴⁸ The most cost-effective way of increasing adiponectin, the major anti-inflammatory adipokine, is reduction of the adipose tissue mass by achieving around 5 to 7% weight loss through hypocaloric diet and physical exercise.^49,50,51 Also, recent studies have shown that GLP-1 agonists, i.e., semaglutide and liraglutide and dual GLP-1 and GIP agonist tirzepatide, can increase adiponectin and reduce pro-inflammatory cytokines, by inducing 10 to 20% weight loss.^52,53 PPAR-gamma agonists such as pioglitazone and rosiglitazone significantly improve insulin sensitivity by increasing adiponectin levels and reducing fetuin-A levels, albeit with loss of weight.^54,55

• 5. Leptin

Leptin is essentially a thinning hormone, inhibiting food intake by stimulating the hypothalamic satiety center. Leptin is secreted majorly from adipocytes. However, small quantities of leptin are secreted from skeletal muscle, liver, placenta, and fundus of the stomach. Leptin levels increase with increasing amount of fat deposition in body. High level of leptin is associated with obesity and T2D.⁵⁶ Leptin resistance leads to unregulated food intake and often co-exists with hypothalamic insulin resistance. The various mechanisms by which a loss of hypothalamic leptin signal leads to weight gain are induction of leptin by Suppressor of Cytokine Signalling-3 (SOC-3), decreased circulation to brain, and increased endoplasmic reticulum (ER) stress. The JAK-STAT pathway activated by leptin receptor is also an important regulator of increasing BMI and phosphoinositol-3-kinase is also regulated by leptin, which, in turn, controls glucose metabolism.⁵⁷ Circulatory leptin levels modulate nutritional status because its level decreases soon after fasting begins.⁵⁸ Leptin secretion is also dependent on body fat content. Also, 80% of total leptin production is from subcutaneous fat. Circulatory leptin levels, and its secretion from adipose tissue are positively correlated with obesity. Higher content of fat is linked to an increase in leptin resistance. Leptin is a crucial moderator in managing body weight. Hence, deficiency of leptin in blood leads to leptin resistance. Leptin resistance is a key player behind adiposity.⁵⁹ Leptin improves insulin sensitivity through AMPK. AMPK in turn decreases malonyl-CoA, thereby impairing acetyl-CoA carboxylase. This explains how leptin deficiency contributes to insulin resistance. It establishes that leptin resistance as a key factor behind adiposity and can be targeted to reduce insulin resistance. Bariatric surgery achieves these goals in a much more robust way, as it allows patients to achieve more than 20% weight loss. DiRECT trial, where participants were subjected to very low-calorie diets (600 calories), resulted in 12 to 15 kg weight loss.^60,61,62,63 This was associated with significant improvement in obesity-associated insulin and leptin resistance, secondary to favorable improvements in adipo-hepatokine profiles. Although the actual part played by leptin in regulating insulin resistance is unclear, the contribution of leptin along with other adipokines such as TNF-α, resistin, IL-6 in MetS is confirmed.⁶⁴ Finally, leptin is a major gate keeper of the initiation of puberty in girl. Adequate leptin levels tell the hypothalamic GnRH neurons that the body has acquired enough adipose tissue store to sustain pregnancy and child birth; the latter then triggers the onset of puberty.⁶⁵

Hepatokines in Metabolic Syndrome

Liver, one of the major glands in human body, plays several functions such as regulating carbohydrate, fat, and protein metabolism. Hepatokines, the liver-derived secretory proteins, have been given the status of ‘new hormones’ having substantial role in MetS. The hepatokines regulate energy homeostasis by controlling glucose and lipid metabolism.⁶⁶ The high prevalence of type 2 diabetes has increased the need for drug development targeting the liver. Many glucose-lowering drugs are used as hepatokine blockers for treating liver diseases. Some known drugs are nuclear hormone receptor agonists such as peroxisome proliferator-activated receptor agonists, and farnesoid X receptor agonists, incretins, fibroblast growth factor-19/21 and sodium–glucose cotransporter inhibitors.⁶⁷ However, detailed studies on deciphering the mechanism is necessary to evaluate hepatokine induced tissue damage and the role of these drugs in metabolic syndrome. Several hepatokines have been identified that provide important knowledge in understanding the pathogenesis of MetS. These hepatokines pose to be the possible targets for treating obesity and other related diseases. The role of all these hepatokines in MetS are as follows and shown in ►Fig. 3:

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Fig. 3

Hepatokines in metabolic syndrome: role of Hepatocyte-derived fibrinogen-related protein 1 (HFREP1) or Fgl1 or hepassocin, Fetuin-A, fibroblast growth factor 21 (FGF-21), angiopoietin-related growth factor (AGF), angiopoietin-related growth factor (AGF), angiopoietin-like proteins (ANGPTL) and leukocyte cell-derived chemotaxin 2 (LECT2) in dyslipidemia, insulin resistance, inflammation, type 2 diabetes, and hypertension.

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• 1. Fetuin-A

Fetuin-A (AHSG), is a liver-derived protein and is one of the major culprits behind T2D via blocking the function of insulin receptor and promoting secretion of cytokines. Fetuin-A reduces adiponectin secretion, in adipocytes and monocytes, which is a main protein that plays important role against MetS. Fetuin-A secretion increases with increased hepatic fat accumulation and sub-clinical atherosclerosis while it is negatively associated with insulin sensitization.⁶⁸ The position of fetuin-A gene is 3q27. Single nucleotide polymorphisms (SNPs) in human AHSG gene is linked to T2D.⁶⁹ The role of excess fetuin-A is reported in obesity, MetS, and T2D. Fetuin-A can be a potential bio-marker for T2D.⁶⁹ Interestingly, plasma fetuin-A levels can be reduced by calorie restriction.⁷⁰ Pioglitazone, an insulin sensitizer, decreases fetuin-A mRNA expression in liver tissue.⁵⁴ Regular exercise or workout has variety of action on circulatory fetuin-A levels; and it has been proven that exercise can reduce serum fetuin-A concentration in T2D while increasing serum adiponectin levels. Lowering fetuin-A level in obese subjects can lead to improvement in IR. Increased serum fetuin-A levels following weight loss after 6 months of aerobic training in healthy overweight old men has been shown to be cardioprotective.⁷¹ Altogether, fetuin-A is a very promising drug target for preventing and treating T2D.⁶⁶

• 2. Hepatocyte-derived fibrinogen-related protein 1 (HFREP1)

Hepatocyte-derived fibrinogen-related protein 1 (HFREP1, or Fgl1 or hepassocin), is produced from the liver and can lead to progression of T2D through ERK1/2. HFREP1 is also secreted in adipocytes where it controls lipid homeostasis. Plasma HFREP1 levels correlate positively with insulin desensitization and the rate of progression of prediabetes to T2D.⁷²

• 3. Fibroblast growth factor 21 (FGF-21)

FGF-21, another hepatokine, is a strong energy regulator and an essential hormone needed for adaptive starvation. FGF21 is also a myokine and an adipokine. It also affects the level of reactive oxygen species (ROS), ER stress, and other cellular processes and exerts a favorable effect on controlling body weight and triglyceride and cholesterol levels. FGF21 increases GLUT-1 expression in the skeletal muscle and insulin-mediated glucose uptake and serum insulin concentrations in diabetes patients.⁷³ LY2405319, an FGF-21 agonist, highly improves lipid content in blood in T2D subjects with direct effect on controlling BMI, circulatory insulin levels, and production of adiponectin. FGF21 regulates metabolic balance by enhancing PPAR-γ actions by inducing the formation of beige or brown adipose tissue from white adipose tissue (WAT).⁷⁴ Browning of WAT is a crucial balance involving two important factors-FGF-21 and irisin. Irisin is another prominent skeletal muscle-derived protein that has a significant role in MetS. Nutrition status has a direct effect on FGF-21 level in MetS. It suppresses the production and function of adiponectin and promotes the secretion of leptin and cytokine IL-6 in maturing adipocytes.^75,76 High-sensitivity C-reactive protein (hsCRP, a popular indicator of inflammatory diseases) has intricate connection with FGF-21 in T2D subjects. Also, transcription and secretion of FGF-21 can be decreased by TNF-α. Circulatory FGF-21 concentrations are also linked to hypertension, making a role of FGF-21 in MetS quite significant. While pre-clinical studies were highly promising in terms of improving insulin sensitivity, the clinical development of FGF-21 as a drug was given up, as human studies showed significant FGF-21 resistance in T2D, undermining its efficacy in clinical T2D.⁷⁷

• 4. Angiopoietin-related growth factor (AGF)

Angiopoietin-related growth factor (AGF) is produced from the liver and has crucial function in maintaining lipids and carbohydrates in our body. AGF deficiency is directly linked to, adiposity, T2D and fat depot formation in various organs. AGF also decreases hepatic gluconeogenesis. Serum AGF level increases in T2D patients and also in MetS patients. AGF increases with progression of T2D, while the level of HDLc in circulation decreases.⁷⁸

• 5. Selenoprotein-P (SeP)

Selenoprotein-P is another crucial hepatokine. High level of SeP is found in T2D or fatty liver patients, or patients having visceral adipocyte accumulation. Such excess concentration of SeP is significantly correlated with IR, hsCRP, and triglyceride level. However, SeP in circulation is low in MetS subjects, particularly those with heart ailments.^79,80 Moreover, excess concentrations of SeP decreases the risk of MetS in children. Adiponectin and salsalate inhibit SeP and this inhibition is mediated by AMPK-Forkhead box protein O1a (FOXO1 a) and its downstream proteins, thereby reducing hepatic glucose output.⁸¹ Serum SeP level shows inverse relation to serum adiponectin concentrations in T2D subjects. SeP can be a potent target for the management of IR-associated diseases.⁸²

• 6. Angiopoietin-like proteins (ANGPTL)

Angiopoietin-like proteins (ANGPTL 1-8), are a group of proteins, secreted from the liver. These have a crucial role in maintaining lipid content in the body. First, we shall discuss about angiopoietin-like protein 4 (ANGPTL4). ANGPTL4 is also secreted from muscle cells and adipocytes. ANGPTL4 primarily regulates the storage of lipid in cells and its spilling. Because the lipid content is directly dependent on food intake, ANGPTL4 secretion is controlled by several factors such as the amount and time of food intake, nutrients present in food, and lifestyle.⁸³ Nutrients in food, particularly fatty acids, can control gene expression of ANGPTL4 via PPARs. ANGPTL4 stimulates lipid breakdown within adipocytes through cAMP. ANGPTL4 is directly associated with IR. Excess production of ANGPTL4 is required to regulate insulin sensitivity; however, sometimes it may lead to excess lipid spillage in circulation and deposition of fat in various organs. ANGPTL4 concentration in circulation decreases in T2D patients, which implies that low level of ANGPTL4 can be a reason behind T2D. AGPTL4 has a key role in maintaining ROS level and inflammation.⁸⁴ ANGPTL8 (also known as lipasin) is another newly studied liver-derived protein that regulates lipid metabolism. ANGPTL8 and HDL-cholesterol have a negative association, while ANGPTL8 and triglyceride level has a positive association. All the effects of ANGPTL8 are moderated by blocking the action of lipoprotein lipase and also ANGPTL3 by increasing the production of very low-density lipoprotein from hepatocytes. The expression of ANGPTL8 is directly related to an increased expression of hsCRP which in turn increases the prevalence of MetS. Plasma ANGPTL8 begin to increase in pre-diabetic patients and continue to increase until the progression of T2D.⁸⁵ ANGPTL8 is also considered to be an indicator of kidney and insulin resistance. Increased secretion of ANGPTL8 occurs in MetS patients with hypertension along with increased expression of ANGPTL4.⁸⁶ So, for future investigations ANGPTLs can be targeted by pharmaceuticals to treat hyperlipidemia.

• 7. Leukocyte cell-derived chemotaxin 2 (LECT2)

Leukocyte cell-derived chemotaxin 2 (LECT2), another hepatokine, is a key protein that can recognize energy demand in our body. It is connected to inflammation in the liver and natural killer T cells. LECT2 is also linked to fat accumulation and glucose transport impairment in skeletal muscles. Positive correlation exists between circulatory LECT2 concentrations, body weight, T2D, and increasing fat depot in the liver and other organs. Calorie-rich diet or fat-containing diet and workout or calorie expenditure exert opposite effects on LECT2 levels, increasing and decreasing its level, respectively, via blocking or activation the AMPK pathway.⁸⁷ LECT2 treatment can increase inflammation by increasing the secretion of cytokines and other inflammatory molecules. LECT2 also increases insulin resistance, accumulation of fat, and phosphorylation of mammalian target of rapamycine (mTOR) in hepatocytes, making its role in MetS prominent.⁸⁸

Conclusion

Taken altogether, adipokines and hepatokines likely contribute to insulin resistance, adiposity, T2D, cardiovascular diseases, dyslipidemia, hypertension, and inflammation, leading to MetS and its sinister consequences. Most adipokines and hepatokines are novel proteins. Several adipokines and hepatokines have crosstalk not only amongst themselves but also with other myokines or organokines. Better knowledge of all these proteins is the need of the hour to help us improve the preventive and therapeutic strategies to be adopted for MetS. The easiest and most effective way to restrain or suppress MetS is by making changes in our mode of lifestyle. Living a healthy life with proper food habits and exercise can delay the onset of MetS or aid in curing MetS along with medications.